Toxicology and Applied Pharmacology

Biography

Biography: Gabriela Hädrich

Abstract

This study evaluates the advantage of the quercetin encapsulation in nanosized emulsion (QU-NE) administered orally in rats in order to demonstrate its anti-oedematous and antioxidant eff ects as well as its toxicity. The nanocarriers were prepared using the hot solvent diffusion with the phase inversion temperature methods. The nanocarriers physicochemical properties were then investigated. The anti-edematous activity was tested using paw edema in rats. In addition, NF-kB expression in subcutaneous tissue of the paws was accessed by immunohistochemistry while the lipid peroxidation was
analyzed in the liver by malondialdehyde reaction with thiobarbituric acid. Hematological, renal and hepatic toxicity as well as the genetic damage were also evaluated. Th e results demonstrated that QU-NE exhibited pronounced anti-oedematous property comparable to drug diclofenac. This eff ect was associated with NF-κB pathway inhibition. Th e lipid peroxidation was also only reduced in rats treated with QU-NE. Besides this, no genetic damage, hematological, renal or hepatic toxicities were observed aft er administration of QU-NE. These results suggest that quercetin nanosized emulsion exhibits anti-oedematous and antioxidant properties and does not demonstrate toxic effects. Th is indicates that it has a potential application in the treatment of inflammatory diseases.