Hanan Osman Ponchet
DMPK Research, France
Title: Characterization and regulation of the expression of drug transporters in human skin
Biography
Biography: Hanan Osman Ponchet
Abstract
Most identified drug transporters belong to ATP-binding cassette (ABC) and solute carrier (SLC) families. It has been recently recognized that like drug metabolizing enzymes, some of drug transporters play an important role in pharmacokinetics and drug exposure and may be involved in clinically relevant drug-drug interactions for systemic drugs. However very little is known about the role of drug transporters in human skin in the disposition of topically applied drugs. Expression profi le of SLC and ABC transporters included in the regulatory guidelines as the most likely clinical sources of drug interactions was characterized in ex vivo human skin using TaqMan real-time RT-PCR. Moreover, the eff ect of rifampicin treatment and solar simulator irradiation on the expression of drug transporters in human skin was investigated as well as the localization of the drug transporters within the diff erent layers of human skin. SLC and ABC transporters have a very specific expression profile in human skin compared to liver and kidney. In addition, expression of ABCC1 (MRP1) and SLC47A1/2 (MATE1 and MATE2) is shown for the fi rst time in human skin. Th e role of drug transporters in drug absorption in human skin will be presented and discussed.
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