Marwa Saad Fathi
Ain Shams University, Egypt
Title: Genotypic and phenotypic patterns of antimicrobial susceptibility of Helicobacter pylori strains among Egyptian patients
Biography
Biography: Marwa Saad Fathi
Abstract
Backgrounds & Study Aim: Helicobacter pylori is currently recognized as one of the most common chronic bacterial infections worldwide. Eradication of bacteria is eff ective in healing peptic ulcers, preventing ulcer relapses and potentially decreasing the risk of progression to gastric carcinoma. For successful eradication of bacteria, it is imperative that the clinician be aware of
the current antimicrobial susceptibility profi les of isolates within the region. Th erefore, the aim of this study is to compare the phenotypic & genotypic patterns of antibiotics susceptibility to Helicobacter pylori strains among Egyptian patients in order to attain a clinical utility from such patterns.
Patients & Methods: 30 symptomatic cases were enrolled. H. pylori infection was diagnosed by upper endoscopy as well as biopsy was taken. Antimicrobial susceptibility to Helicobacter pylori strains was assessed in all subjects by disc diff usion & e-testing methods. Further molecular characterization for genes encoding antimicrobial resistance of isolated strains was done.
Results: For metronidazole, amoxicillin and ciprofl oxacin, we compared the phenotypic and genotypic patterns of resistance as detected by PCR amplifi cation of the resistance genes. E test results were 100%, 50% & 87.5% for metronidazole, ciprofloxacin & amoxicillin respectively from 16 isolated H. pylori strains.
Conclusion: Improving the knowledge of resistance mechanisms, the elaboration of rational and efficacious associations for the treatment H. pylori infection are of high importance especially in determining the therapeutic outcome. Further progress should ultimately focus on the establishment of a cheap, feasible and reliable laboratory test to predict the outcome of a therapeutic scheme