Michał H Wróbel
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Poland
Title: Glyphosate and its most popular product (Roundup) affect the secretion of myometrial contractions regulators from bovine ovary and uterus in vitro
Biography
Biography: Michał H Wróbel
Abstract
Glyphosate based herbicides are intensively used in modern agriculture. Therefore, the aim of the study was to investigate the effect of the pure glyphosate and its most popular product (Roundup) on hormonal regulation of the force of myometrial contractions. The myometrial, endometrial, granulosa and luteal cells, as well as the strips of myometrium, from non-pregnant cows, were incubated with the herbicides at doses (0.1, 1 or 10 ng/ml) which were closed to their environmental amount. Neither glyphosate nor Roundup affected the viability of studied cells. Glyphosate stimulated the secretion of testosterone (T) and estradiol from granulosa cells, while Roundup inhibited the T secretion. Both compounds decreased the secretion of progesterone while increased oxytocin (OT) secretion from luteal cells. Roundup significantly decreased prostaglandins (PGF2 and PGE2) secretion from endometrial but not myometrial cells. However, neither basal nor OT-stimulated myometrial contractions were affected by both compounds. The data show that the herbicides impaired the secretory function of ovarian and endometrial cells. Admittedly both compounds change the amount of regulators of uterine contractions, but they do not affect the force of myometrial contractions directly. The data clearly show that these herbicides can support the luteolytic process but may only indicate the potential of these compounds to disturb the regulation of smooth muscle motility.
Recent Publications
1. Wróbel M H, MÅ‚ynarczuk J, Kotwica J (2012) The effect of DDT and its metabolite (DDE) on prostaglandin secretion from epithelial cells and on contractions of the smooth muscle of the bovine oviduct in vitro. Toxicology and Applied Pharmacology 259(2): 152-159.
2. Wróbel M H, Bedziechowski P, MÅ‚ynarczuk J, Kotwica J (2014) Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH. Environmental Research 132: 54-61.
3. Wrobel M H, Grzeszczyk M, Mlynarczuk J, Kotwica J (2015) The adverse effects of aldrin and dieldrin on both myometrial contractions and the secretory functions of bovine ovaries and uterus in vitro. Toxicology and Applied Pharmacology 285: 23-31.
4. Wrobel M H, Mlynarczuk J (2017) Secretory function of ovarian cells and myometrial contractions in cow are affected by chlorinated insecticides (chlordane, heptachlor and mirex) in vitro. Toxicology and Applied Pharmacology 314: 63-71.
5. Wrobel M H, Mlynarczuk J (2017) The inhibition of myometrial contractions by chlorinated herbicides (atrazine and linuron), and their disruptive effect on the secretory functions of uterine and ovarian cells in cow, in vitro. Pesticide Biochemistry and Physiology DOI: 10.1016/j.pestbp.2017.01.002.
