Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series : World's leading Event Organizer


Semir Gul

Semir Gul

Inonu University, Turkey

Title: Protective role of Vitamin-E over the acetamiprid toxicity in mice testis


Biography: Semir Gul


Statement of the Problem: Acetamiprid (ACMP) is an insecticide of the neonicotinoid class, widely used for combating insects in many areas of agricultural production. Neonicotinoids act as agonists of acetylcholine in the synaptic range and inhibit synaptic stimulation by binding to the nicotinic acetylcholine receptors in the central nervous system of insects. Since different nAChRs subspecies are expressed in human testes, prostate, mammalian nervous system, mouse testes and spermatozoa, these organs are the main targets of neonicotinoids and therefore acetamiprid. It has been reported that the insecticides cause structural and functional damage to spermatozoon, Sertoli cells and Leydig cells by oxidative stress in the testis. Vitamin-E is a cell membrane soluble vitamin and plays a protective role against harmful effects of free radicals in cells and tissues with antioxidant property. It is known that Vitamin-E is a potent lipophilic antioxidant that is vital for the protection of mammalian spermatogenesis and is present in high amounts in Sertoli and spermatogenic cells. We propose that the use of vitamin-E against acetamiprid testis toxicity may play protective role through oxidant-antioxidant mechanism.


Methodology & Theoretical Orientation: Thirty mice were divided into 5 groups (n=6 for each): water, corn oil, ACMP (25mg/kg/day), Vit-E (100mg/kg/day), ACMP+Vit-E (25mg/kg/day ACMP and 100mg/kg/day Vit-E). After 21 days, testis tissues were taken for histological, biochemical and intracellular ultrastructural evaluations.


Findings: In water, corn oil and Vit-E groups, testes tissues had normal testicular histomorphology, mature-healthy spermatogenic cells and seminiferous tubules. In ACMP group, degenerations in germ cells that accumulated in the lumen of the seminiferous tubule, seminiferous tubular atrophy and organization disorder of seminiferous tubule epithelium were observed. Histomorphological damage changes in the ACMP+Vit-E group were less than in ACMP group. However, histological scores showing the tissue improvement in ACMP+Vit-E were not very close to water, corn oil and Vit-E groups.