Hatem Ahmed
Naif Arab University for Security Sciences, Saudi Arabia
Title: Estimation of Pb and Cd in the whole blood by ICP-OES technique: A comparison of microwave digestion and simple dilution method
Biography
Biography: Hatem Ahmed
Abstract
Forensic investigations necessitate the quick identification and quantification of toxic substances to begin adequate treatment for trace metal toxicity. As a result, finding precise and time-effective digestive methods for identifying toxic substances in biological samples of a person suspected of poisoning is critical. In the present study, a comparison between the microwave digestion and simple dilution method of blood samples and their detection by induction coupled optical emission spectroscopy ICP-OES was carried out. Twenty-three human whole blood samples were collected from fully consented volunteers in EDTA-blood tubes. Whole blood samples were prepared using HNO3:H2O2 for microwave digestion and HNO3:H2O2, Triton X100, and N-butanol for the simple dilution method. Validation of the method was executed by determining accuracy and precision. Method sensitivity was assessed by determining limits of detection (LOD) and quantification (LOQ). Our results showed a significant difference in the recovery ratios in favor of lead (Pd) and cadmium (Cd) between simple dilution and microwave digestion techniques. This study indicated that microwave digestion may be a better choice than the simple dilution technique in terms of recovery and accuracy whereas simple dilution was both cost and time-effective for the isolation, identification, and quantification of toxic metals in cases of poisoning.