Quinteros M A
National Scientific and Technical Research Council (CONICET), Argentina
Title: Biosynthesized silver nanoparticles: Decoding their mechanism of action in S. aureus and E. coli
Biography
Biography: Quinteros M A
Abstract
The generation of oxidative stress in bacteria in the presence of silver nanoparticles (AgNPs) is already widely known. If the cell cannot respond to oxidative injury produced by increased species reactive oxygen (ROS), the oxidation of macromolecules such as proteins, lipids and DNA occurs, leading to the death of the bacterium. In previous results, we observed as biosynthesized AgNPs that had antibacterial activity, generated an increase of ROS and RNI in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, being responsible for their toxicity and bacterial death. According to the above, we set out to delve into the mechanism of action of AgNPs, by determining markers of oxidative stress, such as protein oxidation, lipids, DNA and changes in membrane potential in two reference strains S. aureus ATCC 29213 and E. coli ATCC 25922. We found that the increase in the levels of ROS is associated with the oxidation of different macromolecules important for the normal functioning of the cell, so that oxidative stress would be one of the mechanisms by which the AgNPs would exert their toxicity in these two strains of great clinical relevance. In this way, we are making a great contribution on the toxicity produced by AgNPs.
Recent Publications
1. Quinteros M A, Aiassa Martínez I M, Dalmasso P R, Páez P L (2016) Silver nanoparticles: Biosynthesis using an ATCC reference strain of Pseudomonas aeruginosa and activity as broad spectrum clinical antibacterial agents. Int. J. Biomater. 5971047.
2. Quinteros M A, Cano Aristizábal V, Dalmasso P R, Paraje M G, Páez P L (2016) Oxidative stress generation of silver nanoparticles in three bacterial genera and its relationship with the antimicrobial activity. Toxicol. In Vitro 36:216-23.
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